FACS:Article Title: Slow integrin-dependent migration organizes networks of tissue-resident mast cells
Article Snippet: Rat Anti-nidogen (NG2); Thermo Fisher; Cat# MA1-06501; 1:300 Donkey Anti-rat Alexa Fluor 405-conjugated; Abcam; Cat# ab175670; 1:500 FACS sort for single-cell RNA sequencing Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated; BioLegend; Cat# 109847; 1:100 Armenian Hamster Anti-CD3e PE-conjugated; Thermo Fisher Scientific; Cat# 2-0031-83; 1:100 Rat Anti-CD4; PE-conjugated BioLegend; Cat# 100407; 1:100 Rat Anti-CD8a PE-conjugated; Thermo Fisher Scientific; Cat# 12-0081-82; 1:100 Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated; BioLegend; Cat# 117307; 1:100 Rat Anti-CD19 PE-conjugated; Thermo Fisher Scientific; Cat# 12-0193-83; 1:100 Mouse Anti-NK-1.1 PE-conjugated; BioLegend; Cat# 108707; 1:100 Rat Anti-F4/80 PE-conjugated; Thermo Fisher Scientific; Cat# MF48004; 1:100 Validation All commercial antibodies, validation informations can be found on the antibody registry (https://antibodyregistry.org) or company websites: Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated: PRID: AB_313776 Armenian Hamster Anti-CD29 (Integrin beta 1) PE-conjugated: PRID: AB_312885 Armenian Hamster Anti-CD3e PE-conjugated: PRID:AB_465496 Armenian Hamster Anti-CD61 (Integrin beta 3) PE-conjugated: PRID: AB_394800 Armenian Hamster Anti-FcεRIα Alexa Fluor 647-conjugated: PRID: AB_1626097 Armenian Hamster Anti-FcεRIα PE/CY7-conjugated; PRID AB_10640122 Armenian Hamster IgG Isotype Control PE-conjugated: PRID: AB_326593 Donkey Anti-goat Alexa Fluor 488-conjugated: PRID: AB_2534102 Rabbit Anti-Red Fluorescent Protein (RFP): PRID:AB_2209751 Donkey Anti-mouse CyTM3-conjugated: PRID: AB_2340813 Donkey Anti-rabbit Alexa Fluor 488-conjugated: PRID: AB_2535792 Donkey Anti-rabbit Alexa Fluor 568-conjugated: PRID: AB_2534017 Donkey Anti-rat Alexa Fluor 405-conjugated: https://www.abcam.com/donkey-rat-igg-hl-alexa-fluor-405-preadsorbedab175670.html Donkey Anti-rat Alexa Fluor 647-conjugated: PRID: AB_2813835 Goat Anti-GFP DylightTM 488-conjugated: RRID: AB_1961516 Goat Anti-Mcpt6: PRID: AB_884330 Goat Anti-mouse Alexa Fluor 647-conjugated: PRID: AB_2535806 Goat Anti-rabbit Alexa Fluor 405-conjugated: PRID: AB_221605 Goat Anti-rat Alexa Fluor 568-conjugated: PRID: AB_2534121 Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated: PRID: AB_2616859 Mouse Anti-Dinitrophenyl (DNP) IgE: Eshhar, Z., Ofarim, M. & Waks, T. Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity.
RNA Sequencing:Article Title: Slow integrin-dependent migration organizes networks of tissue-resident mast cells
Article Snippet: Rat Anti-nidogen (NG2); Thermo Fisher; Cat# MA1-06501; 1:300 Donkey Anti-rat Alexa Fluor 405-conjugated; Abcam; Cat# ab175670; 1:500 FACS sort for single-cell RNA sequencing Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated; BioLegend; Cat# 109847; 1:100 Armenian Hamster Anti-CD3e PE-conjugated; Thermo Fisher Scientific; Cat# 2-0031-83; 1:100 Rat Anti-CD4; PE-conjugated BioLegend; Cat# 100407; 1:100 Rat Anti-CD8a PE-conjugated; Thermo Fisher Scientific; Cat# 12-0081-82; 1:100 Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated; BioLegend; Cat# 117307; 1:100 Rat Anti-CD19 PE-conjugated; Thermo Fisher Scientific; Cat# 12-0193-83; 1:100 Mouse Anti-NK-1.1 PE-conjugated; BioLegend; Cat# 108707; 1:100 Rat Anti-F4/80 PE-conjugated; Thermo Fisher Scientific; Cat# MF48004; 1:100 Validation All commercial antibodies, validation informations can be found on the antibody registry (https://antibodyregistry.org) or company websites: Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated: PRID: AB_313776 Armenian Hamster Anti-CD29 (Integrin beta 1) PE-conjugated: PRID: AB_312885 Armenian Hamster Anti-CD3e PE-conjugated: PRID:AB_465496 Armenian Hamster Anti-CD61 (Integrin beta 3) PE-conjugated: PRID: AB_394800 Armenian Hamster Anti-FcεRIα Alexa Fluor 647-conjugated: PRID: AB_1626097 Armenian Hamster Anti-FcεRIα PE/CY7-conjugated; PRID AB_10640122 Armenian Hamster IgG Isotype Control PE-conjugated: PRID: AB_326593 Donkey Anti-goat Alexa Fluor 488-conjugated: PRID: AB_2534102 Rabbit Anti-Red Fluorescent Protein (RFP): PRID:AB_2209751 Donkey Anti-mouse CyTM3-conjugated: PRID: AB_2340813 Donkey Anti-rabbit Alexa Fluor 488-conjugated: PRID: AB_2535792 Donkey Anti-rabbit Alexa Fluor 568-conjugated: PRID: AB_2534017 Donkey Anti-rat Alexa Fluor 405-conjugated: https://www.abcam.com/donkey-rat-igg-hl-alexa-fluor-405-preadsorbedab175670.html Donkey Anti-rat Alexa Fluor 647-conjugated: PRID: AB_2813835 Goat Anti-GFP DylightTM 488-conjugated: RRID: AB_1961516 Goat Anti-Mcpt6: PRID: AB_884330 Goat Anti-mouse Alexa Fluor 647-conjugated: PRID: AB_2535806 Goat Anti-rabbit Alexa Fluor 405-conjugated: PRID: AB_221605 Goat Anti-rat Alexa Fluor 568-conjugated: PRID: AB_2534121 Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated: PRID: AB_2616859 Mouse Anti-Dinitrophenyl (DNP) IgE: Eshhar, Z., Ofarim, M. & Waks, T. Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity.
Biomarker Discovery:Article Title: Slow integrin-dependent migration organizes networks of tissue-resident mast cells
Article Snippet: Rat Anti-nidogen (NG2); Thermo Fisher; Cat# MA1-06501; 1:300 Donkey Anti-rat Alexa Fluor 405-conjugated; Abcam; Cat# ab175670; 1:500 FACS sort for single-cell RNA sequencing Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated; BioLegend; Cat# 109847; 1:100 Armenian Hamster Anti-CD3e PE-conjugated; Thermo Fisher Scientific; Cat# 2-0031-83; 1:100 Rat Anti-CD4; PE-conjugated BioLegend; Cat# 100407; 1:100 Rat Anti-CD8a PE-conjugated; Thermo Fisher Scientific; Cat# 12-0081-82; 1:100 Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated; BioLegend; Cat# 117307; 1:100 Rat Anti-CD19 PE-conjugated; Thermo Fisher Scientific; Cat# 12-0193-83; 1:100 Mouse Anti-NK-1.1 PE-conjugated; BioLegend; Cat# 108707; 1:100 Rat Anti-F4/80 PE-conjugated; Thermo Fisher Scientific; Cat# MF48004; 1:100 Validation All commercial antibodies, validation informations can be found on the antibody registry (https://antibodyregistry.org) or company websites: Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated: PRID: AB_313776 Armenian Hamster Anti-CD29 (Integrin beta 1) PE-conjugated: PRID: AB_312885 Armenian Hamster Anti-CD3e PE-conjugated: PRID:AB_465496 Armenian Hamster Anti-CD61 (Integrin beta 3) PE-conjugated: PRID: AB_394800 Armenian Hamster Anti-FcεRIα Alexa Fluor 647-conjugated: PRID: AB_1626097 Armenian Hamster Anti-FcεRIα PE/CY7-conjugated; PRID AB_10640122 Armenian Hamster IgG Isotype Control PE-conjugated: PRID: AB_326593 Donkey Anti-goat Alexa Fluor 488-conjugated: PRID: AB_2534102 Rabbit Anti-Red Fluorescent Protein (RFP): PRID:AB_2209751 Donkey Anti-mouse CyTM3-conjugated: PRID: AB_2340813 Donkey Anti-rabbit Alexa Fluor 488-conjugated: PRID: AB_2535792 Donkey Anti-rabbit Alexa Fluor 568-conjugated: PRID: AB_2534017 Donkey Anti-rat Alexa Fluor 405-conjugated: https://www.abcam.com/donkey-rat-igg-hl-alexa-fluor-405-preadsorbedab175670.html Donkey Anti-rat Alexa Fluor 647-conjugated: PRID: AB_2813835 Goat Anti-GFP DylightTM 488-conjugated: RRID: AB_1961516 Goat Anti-Mcpt6: PRID: AB_884330 Goat Anti-mouse Alexa Fluor 647-conjugated: PRID: AB_2535806 Goat Anti-rabbit Alexa Fluor 405-conjugated: PRID: AB_221605 Goat Anti-rat Alexa Fluor 568-conjugated: PRID: AB_2534121 Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated: PRID: AB_2616859 Mouse Anti-Dinitrophenyl (DNP) IgE: Eshhar, Z., Ofarim, M. & Waks, T. Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity.
Control:Article Title: Slow integrin-dependent migration organizes networks of tissue-resident mast cells
Article Snippet: Rat Anti-nidogen (NG2); Thermo Fisher; Cat# MA1-06501; 1:300 Donkey Anti-rat Alexa Fluor 405-conjugated; Abcam; Cat# ab175670; 1:500 FACS sort for single-cell RNA sequencing Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated; BioLegend; Cat# 109847; 1:100 Armenian Hamster Anti-CD3e PE-conjugated; Thermo Fisher Scientific; Cat# 2-0031-83; 1:100 Rat Anti-CD4; PE-conjugated BioLegend; Cat# 100407; 1:100 Rat Anti-CD8a PE-conjugated; Thermo Fisher Scientific; Cat# 12-0081-82; 1:100 Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated; BioLegend; Cat# 117307; 1:100 Rat Anti-CD19 PE-conjugated; Thermo Fisher Scientific; Cat# 12-0193-83; 1:100 Mouse Anti-NK-1.1 PE-conjugated; BioLegend; Cat# 108707; 1:100 Rat Anti-F4/80 PE-conjugated; Thermo Fisher Scientific; Cat# MF48004; 1:100 Validation All commercial antibodies, validation informations can be found on the antibody registry (https://antibodyregistry.org) or company websites: Armenian Hamster Anti-CD11c (Integrin alpha X)PE-conjugated: PRID: AB_313776 Armenian Hamster Anti-CD29 (Integrin beta 1) PE-conjugated: PRID: AB_312885 Armenian Hamster Anti-CD3e PE-conjugated: PRID:AB_465496 Armenian Hamster Anti-CD61 (Integrin beta 3) PE-conjugated: PRID: AB_394800 Armenian Hamster Anti-FcεRIα Alexa Fluor 647-conjugated: PRID: AB_1626097 Armenian Hamster Anti-FcεRIα PE/CY7-conjugated; PRID AB_10640122 Armenian Hamster IgG Isotype Control PE-conjugated: PRID: AB_326593 Donkey Anti-goat Alexa Fluor 488-conjugated: PRID: AB_2534102 Rabbit Anti-Red Fluorescent Protein (RFP): PRID:AB_2209751 Donkey Anti-mouse CyTM3-conjugated: PRID: AB_2340813 Donkey Anti-rabbit Alexa Fluor 488-conjugated: PRID: AB_2535792 Donkey Anti-rabbit Alexa Fluor 568-conjugated: PRID: AB_2534017 Donkey Anti-rat Alexa Fluor 405-conjugated: https://www.abcam.com/donkey-rat-igg-hl-alexa-fluor-405-preadsorbedab175670.html Donkey Anti-rat Alexa Fluor 647-conjugated: PRID: AB_2813835 Goat Anti-GFP DylightTM 488-conjugated: RRID: AB_1961516 Goat Anti-Mcpt6: PRID: AB_884330 Goat Anti-mouse Alexa Fluor 647-conjugated: PRID: AB_2535806 Goat Anti-rabbit Alexa Fluor 405-conjugated: PRID: AB_221605 Goat Anti-rat Alexa Fluor 568-conjugated: PRID: AB_2534121 Mouse Anti-CD45.2 Brilliant Violet 711TM-conjugated: PRID: AB_2616859 Mouse Anti-Dinitrophenyl (DNP) IgE: Eshhar, Z., Ofarim, M. & Waks, T. Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity.
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